Design and analysis of quantitative PCR experiments for laboratory scientists and statisticians

Category: Laboratory Studies

Friday 3^rd September 10am to 12pm

Recording of event – please click here

The aim of the NIHR Laboratory Statistics Group is to bring together NIHR Statistician and Laboratory Scientists to ensure the best methods are used. We are embarking on a series of workshops to help bridge the divide. The first session looks at the use of quantitative PCR from the laboratory and analysis perspective.

Laboratory Scientists and Statisticians are welcome.

Abstract

This workshop will cover the design and analysis of quantitative PCR (qPCR) experiments, aimed both at lab researchers and statisticians. This will include:

• Introduction to qPCR and its uses.
• qPCR chemistry and how it can be used for quantification.
• Experimental setup, reference genes and controls.
• Calculation of relative expression – ddCT values and fold differences.
• Analysis considerations for relative expression – scale, normality assumptions and tests.
• Graphical representation of qPCR results.
• Statistical considerations for the design of robust qPCR experiments – units of analysis and sample size calculations.

 Register in advance for this meeting:

https://bham-ac-uk.zoom.us/meeting/register/tZcscOqrqjItGNPQgLxfjv32eY0PCEpaG1z0

After registering, you will receive a confirmation email containing information about joining the meeting.