Design and analysis of quantitative PCR experiments for laboratory scientists and statisticians

Location: Online | Date: 3 September 2021

10am to 12pm

The aim of the NIHR Laboratory Statistics Group is to bring together NIHR Statisticians and Laboratory Scientists to ensure the best methods are used. We are embarking on a series of workshops to help bridge the divide. The first session looks at the use of quantitative PCR from the laboratory and analysis perspective.

Laboratory Scientists and Statisticians are welcome.

Abstract

This workshop will cover the design and analysis of quantitative PCR (qPCR) experiments, aimed both at lab researchers and statisticians. This will include:

·        Introduction to qPCR and its uses.

·        qPCR chemistry and how it can be used for quantification.

·        Experimental setup, reference genes and controls.

·        Calculation of relative expression – ddCT values and fold differences.

·        Analysis considerations for relative expression – scale, normality assumptions and tests.

·        Graphical representation of qPCR results.

·        Statistical considerations for the design of robust qPCR experiments – units of analysis and sample size calculations.

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